Laboratory-based Short Course (2)
Course Duration: 7 days
Maximum Class Size: 10
Topics:
(i) Sample handling. The segment introduces participants to a wide array of possible methods for sampling. Design, selection, handling and management of samples are the critical steps in the overall procedures of molecular methodology. The choice of specific laboratory technique determines the appropriate procedures for sampling. A key issue is the extraction of nucleic acids. Related protocols will be presented and discussed.
Course Units: 2 hours.(ii) Enzymes used in molecular biology. The segment covers mechanisms of action, in vivo and in vitro, applications and appropriate modes of use as well as correct handling of enzymes.
Course Units: 2 hours.(iii) Electrophoresis. The segment reviews the theory and applications of the various types of electrophoresis (mainly of nucleic acids but also of proteins).
Course Units: 2 hours.(iv) Cloning. This segment introduces guidelines for cloning. The focus is on the principles of cloning, for research purposes and laboratory use. Further topics include: cloning of a DNA fragment, cloning of PCR products, library construction and methods for identification of desired clones.
Course Units: 4 hours.v) Sequencing. This segment covers different methods for the sequencing of a DNA fragment.
Course Units: 2 hours.(vi) PCR. This covers the theory of the polymerase chain reaction (PCR). (Participants needing the in-depth study and practice of PCR techniques should then take Course 3 – PCR).
Course Units: 3 hours.(vii) Laboratory practice. Several selected protocols will be applied in the lab, covering the subjects presented above, namely, nucleic acid extraction, cloning and PCR. The use of modifying and restriction enzymes as well as electrophoresis is part of the above processes and will be demonstrated too. Throughout the laboratory practice, participants will be actively involved in the various steps of the protocols.
Total time: 20 hours.